
Luciana Pastena Giorno
Federal University of ABC, BrazilTitle: Jet-spinning technique applied in skin tissue engineering: proposal of a PCL/Gelatin polimeric device
Abstract
Introduction: Concepts from materials science
and biology have been studied to develop a
material suitable for burns and rare skin diseases.
Both the raw material and the technique used to
produce a biomedical material must be
considered. As the material impacts cellular
interaction, in addition to stimulating a possible
inflammatory response of the patient's body, it
was proposed to develop a material based on
low-cost bioabsorbable and biocompatible
polymers for these tissue injuries.
Objective: To
verify the material's characteristics, and cellular
interaction in the biomaterial.
Materials and
Method: Polycaprolactone fibers were formed by
Jet-spinning technique, and their surface was
modified with gelatin. Mechanical testing, fiber
porosity, thermal properties, and scanning electron
microscopy were performed.
Results and
Discussion: Differential scanning calorimetry
showed that the gelatin reduced the melting point
and degree of crystallinity polycaprolactone
fibers, in addition to indicating greater elastomeric
properties. In general, the thermograms were in
agreement with the literature. The data showed
that the biomaterial was stable at room
temperature and under commonly used storage
conditions.
The porosity correlates the cell-material
interactions, and the gelatin increased cellular
interactivity to polycaprolactone, as well as
indicated secretory phenotype for the cells. On the
surface of the material, well-spread and adherent
cells were observed, with vesicles or microvilli on
them.
Conclusion: Both the material and the
technique used were promising. Furthermore, the
material structure can be changed by the polymer
type, polymer concentration and/or solvents.
Biography
Visiting researcher at the Brazilian Center of
Reference in Technological Innovations for
Paralympic Sport, Brazil. Ph.D. in progress at the
Federal University of ABC. She has been serving
as an editorial board member of journals, and as a
reviewer. Master's degree in Biotechnology
(UFABC, 2020). In the same period she had an
honorable mention to participate as a
co-supervision of academic work on tissue
engineering for skeletal muscle injuries.
Residency in Organ Transplantation and
Harvesting (UNIFESP, 2015). Worked as a
Physiotherapist at Hospital do Coração/São Paulo,
focusing on intensive care units, quality of life,
pre and postoperative period, and kidney/heart
transplants. Participated in the organizing
committee of the 1st International Workshop
Lolita San Miguel, Brazil (FEFISA, 2012).