Mohammad Sadeq Al-AwarAl-Razi University and Amran University, Yemen
Title: Anti-diabetic activities of Zizyphus spina-christi seeds embryos extract on general characteristics of diabetes, carbohydrate metabolism enzymes and lipids profile in rats
This study was aimed at evaluating the anti-diabetic activities of Zizyphus spina-christi seeds embryos extract (ZSC seeds embryos extract) on general characteristics of diabetes, carbohydrate metabolism enzymes, and lipids profile in alloxan-induced diabetic rats. A single injection of alloxan to rats (150mg/kg) caused pathological alterations in all studied parameters and histological structures of the pancreas. Treatment of diabetic rats with ZSC seeds embryo extract normalized this alteration. Diabetic rats were treated with ZSC seeds embryos extract (2doses; 200&400 mg/kg) and glibenclamide (0.6 mg/kg b.w) for 6 weeks. Fasting blood glucose levels were measured on zero time, 4 h, 8 h and 24 h. Also serum glucose level and body weight were measured every week for all rats. Results showed that oral administration of ZSC seeds embryo extract caused a significant reduction in blood glucose level and plasma lactate together with significant elevation in serum insulin, and serum pyruvate with normalization in body weight. Marked elevation in total antioxidant capacity with lowering of percentage of HbA1C % was reported. In line with amelioration of the diabetic state, ZSC seeds embryos extract restored hepatic and muscle glycogen content and hexokinase activity together with a significant decrease of glucose-6-phosphatase and fructose 1, 6 biphosphatase enzymes activity. Moreover, the extract succeeded to reduce the elevated serum total cholesterol, triglyceride and LDL levels and elevating the reduced HDL level of diabetic rats. This study for the first time reveals that ZSC seeds embryos extract, showed marked amelioration in diabetic rats by stimulating of insulin release from the remnant ?-cells, inhibiting glucagon secretion from alpha-cells, and increase of antioxidant mechanisms, as well as attenuation of meal-derived glucose absorption. This may be attributed to the presence of saponin glycosides, polyphenols, flavonoids and terpenoids which phytochemical screening revealed this presence in ZSC seeds embryos extracted in this study.
Mohammed Al-Awar completed My Ph.D. at the age of 30 years from Sanaa University, Yemen. He is the head of mechanical engineering department of Stanford University, USA. I have over 35 publications that have been cited over 145 times, and his publication h-index is 5. I have been serving as an editorial board member of several reputed journals.