Virtual Conference
Pharma Conference 2022

Neelam Iftikhar

Government College University Faisalabad, Pakistan

Title: Effects of polyphenol-rich traditional herbal teas on obesity and oxidative stress in rats fed a high-fat-sugar diet

Abstract

The present research work was planned to investigate the potential of polyphenol rich extracts of Hibiscus rosa-sinensis (HRS) and Zingiber officinalis (ZO) teas against the obesity and related biochemical parameters of high-fat-sugar diet-induced obesity rats. Three herbal teas were prepared from HRS flowers and ZO rhizomes and their mixture (HRS:ZO, 3:1). Extracts were prepared and total reduced capacity (TRC) and total flavonoid contents (TFC) of the extracts were estimated as gallic acid and catechin equivalents (GAE and CE), respectively. TRC of HRS and ZO extracts were found to be 5.82 and 1.45 mg/g of dry plant material, measured as GAE while TFC were 9.17 and 1.95 mg/g of dry plant material, as CE, respectively. Reverse Phase-HPLC analysis revealed the presence of 15 phenolic acids and 4 flavonoids in herbal extracts of both samples. Catechin, rutin, gallic acid, 4-hydroxy benzoic acid, chlorogenic acid, caffeic acid and salicylic acid were the major compounds detected. 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay was performed and both extracts showed > 50% DPPH radical scavenging capacity. Two levels (250 and 500 mg/kg BW) of each tea were selected to assess anti-obesity potential using high-fat-sugar-diet-induced obesity rat’s model. Data showed that higher dose of HRS significantly reduced the rat’s body weight and body mass index as compared to high-fat-sugar diet group. Total cholesterol, high and low density lipoproteins, triglycerides, kidney, liver and atherogenic indexes, bilirubin total, aspartate aminotransferase, alanine aminotransferase, alkaline phosphate and serum cretanine of rats showed that HRS extract showed significant anti-obesity potential. Moreover, HRS extract also prevent the alterations in malondialdehyde, superoxide dismutase and reduced glutathione levels of experimental rats, thus also showed potential against oxidative stress. It is evident from the results that higher dose of HRS exhibited best protective effects against obesity and oxidative stress while ZO showed fewer protective effects.

Biography

In 2017, I managed to obtain my BS Degree in Analytical Chemistry from the University of Sarghoda, Pakistan. During my BS study, I have studied various analytical, organic and biochemistry courses. I obtained my M.Phil Analytical Chemistry from the Government College University Faisalabad in 2019. Currently I am a student of PhD Chemistry and I have completed the all departmental requirements. My area of interest is Bioanalytical Chemistry.  Major courses I have studied were of Chemistry discipline including chromatographic techniques, nuclear medicine, electro analytical techniques, and thermal methods of analysis. I have studied both theoretical and practical courses. I have broad spectrum knowledge in the area of natural product chemistry. I proved myself to be extremely productive and published two research papers in international journals. I have presented my research work in International conference. I am also good in handling of state of the art chromatographic and spectroscopic techniques. I am not only an intelligent and hardworking researcher, capable of excellent performance, but also a versatile person participating in various extracurricular and social activities. I am an active member of student societies and I got Laptop prize from Chief Minister of Punjab under Youth Punjab Laptop Scheme-2014.