Virtual Conference
Pharma Conference 2022

Yisel González-Madariaga

Villa Clara University of Medical Sciences, Cuba

Title: Effect on liver and kidney of extracts rich in steroidal saponins from Agave brittoniana Trel in an experimental metabolic syndrome model


Introduction: Previous studies have shown the lipid-lowering and anti-inflammatory effect of Agave brittoniana T species in a model of experimental metabolic syndrome (EMS). On the other hand, it is known that the liver and kidneys are affected organs in this metabolic condition.

Objective: To evaluate the possible effect at the tissue level caused by the treatment with products extracted from the species in an EMS model. Materials and methods: EMS was obtained by supplying 35% sucrose for 20 weeks to recently weaned male Wistar rats. Subsequently, doses of 400 mg/kg of hydroalcoholic extract, 100 mg/kg of butanolic fraction, as well as 25, 50 and 100 mg/kg of crude sapogenins were evaluated. Metformin and nicotinic acid were used as controls. Liver and kidney samples were extracted after 14 days of treatment.

Results: The presence of micro and macrovacuolar hepatic steatosis was confirmed in animals with untreated EMS. Hydroalcoholic and butanolic extracts, as well as low and medium doses of sapogenins, decreased this sign. In the group with the highest dose of sapogenins, the appearance of the hepatocytes was normal. Marked arteriolar thickening with extracellular matrix deposition and decreased lumen was observed in EMS without treatment. Doses of hydroalcoholic extract, butanolic extract, and medium and high doses of sapogenins notably improved this thickening, with the best results being observed in the latter.

Conclusions: The hydroalcoholic and butanolic extract, as well as the high dose of sapogenins, achieve tissue-level results similar to those of the healthy group.


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